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Information on the latest vitamin D news and research.

Find out more information on deficiency, supplementation, sun exposure, and how vitamin D relates to your health.

Vitamin D Test Kits


Purchase a test kit


What is the in-home vitamin D test kit?

The Vitamin D Council in-home vitamin D test kit is an easy, affordable and accurate way to find out your vitamin D status and determine if you are getting enough vitamin D.

The Vitamin D Council has partnered with Heartland Assays to bring the best home-testing solution on the market, the cheapest available in the United States.

The convenience of an in-home test kit, coupled with the affordability offered by the Vitamin D Council test kit, means it is easier now more than ever to know if you are vitamin D deficient and need to raise your levels or maintain them if they are in the healthy range.

The Vitamin D Council in-home vitamin D test kit can be used to measure vitamin D levels in both adults and children.

What if I’m a Vitamin D Council monthly, yearly or lifetime member?

Monthly, yearly and lifetime members of the Vitamin D Council are eligible for a discounted price on our in-home test kits! If you have a subscription with the Vitamin D Council, use your login details when ordering the test kit to receive the discounted price. Click here for more information about Vitamin D Council memberships.

How it works

  1. After ordering your test kit and creating an account (or using your existing Vitamin D Council membership), it will be shipped right to your door.
  2. Included in the test kit is a blood spot card with unique ID number, two lancets, an alcohol prep pad, a sterile gauze pad, bandage, and a return envelope and label.
  3. Read the instruction card included in the test kit and follow each step carefully to ensure proper blood spot collection. You can also watch our instructional video for additional clarification.
  4. Make note of your unique ID number so you can easily find your results when they are ready.
  5. Do NOT write your name on the blood spot card or return envelope. Heartland Assays will not be able to process your order if your name is on any of the materials returned to the lab.
  6. Once you’ve completed the spot collection, mail your spot card using the return envelope. If you live in the United States, you don’t need to pay any return shipping costs.
  7. Please allow 5 business days from when you send your spot card for Heartland Assays to receive it.
  8. When Heartland Assays receives your spot card, you will receive an email within 2-3 weeks notifying you that your results are ready.
  9. Log in to your account on the Vitamin D Council website to see your results.

What do your results mean?

The Vitamin D Council has several articles on vitamin D testing and on interpreting results. For more information, please see these pages:

Please contact the Vitamin D Council with any further questions by emailing us at info@vitamindcouncil.org or calling 1-805-439-1075.

Who is Heartland Assays?

Heartland LogoHeartland Assays, LLC is a small midwest company located in Ames, Iowa and is excited to be partnering with the Vitamin D Council in providing affordable vitamin D blood spot testing to its readers, subscribers and the general public.

Heartland Assays also provides analytical services to researchers and clinicians particularly in the area of vitamin D and calcium metabolism. With access to cutting edge technology and over 60 years of combined experience of its principals, Dr. Ron Horst and Dr. Bruce Hollis, Heartland Assays has the knowledge, skills and expertise to provide a one-stop shop for most vitamin D analytical needs.

Heartland Assays is CLIA and CAP certified and participates in the DEQAS proficiency testing scheme.

Please visit Heartland Assay’s website for more information and a full list of services www.heartlandassays.com.


  • By ordering the Vitamin D Council in-home test, I agree and understand that I have not entered into a doctor-patient relationship with Executive Director of the Vitamin D Council, Dr. John Cannell, who provides the doctor’s order for the test.
  • I understand that Dr. John Cannell is not my doctor.
  • I understand that the test kit will report my 25(OH)D level. The results of this test are strictly for reference, not for any diagnostic purposes.
  • California disclaimers regarding testing do not apply.
  • State health laws prohibit the test of specimens collected in or mailed from New York and Maryland and prohibits the transmission of data from Heartland Assays to New York and Maryland physicians or residents. The Vitamin D Council and Heartland Assays are not able to process these orders.
Shipping & Return Policies
Orders typically ship within two business days but date and time of order shipment or delivery is not guaranteed. Shipments to PO boxes may experience delays. At this time, the Vitamin D Council cannot accept or ship orders to outside of the United States. NY and MD residents: Due to special restrictions, no orders will be shipped to the state of NY or MD. Estimated delivery times do not include weekends or holidays. Shipping fees are non-refundable. The original test kit, including all contents must be returned to Vitamin D Council before any refunds can be issued. To be eligible for a refund, your test kit must be returned to the Vitamin D Council within 40 days of purchase.

6 Responses to Vitamin D Test Kits

  1. rjudkins says:

    Are these one use only? Can additional testing cards be purchased for less than the $50 test kit cost?

  2. Jeff Nicklas says:

    Yes, the spot cards are one use only.

    If you wish to purchase more than one kit, the price of the four pack equals less than $50/kit. Otherwise, you will need to purchase the kits at the $50 price.


  3. Louise says:

    If a 4-pack is purchased, is there a shelf life to the spot cards?
    Thank you in advance for your response! And for maintaining your high standard of informative newsletters.
    Aloha, Louise

  4. Jeff Nicklas says:


    The spot cards have a general shelf-life of about 3 years.

    If you plan to keep your test kits long-term, you will want to keep in mind that the spot card can become unusable if exposed to soil, heat or contamination (oils, dirt, etc.s).

    It is important that you keep the flap tucked in on the spot card to protect the filter paper, and that the spot card (and entire kit) is kept in a cool, dry place out of direct sunlight.


  5. varus says:

    This kit did not work out for me. I used one lancet on the side of my non-dominant hand’s middle finger and could not get enough blood to fill even one circle. Lightly stroking, harder stroking then as a last resort going against instructions and squeezing the finger didn’t produce a flow of blood. Same thing on ring finger. Bottom line, lancet pricks on a finger do not produce enough blood. $50 in the garbage.

    • naturesfood93786000 says:

      Try having a hot shower first to warm yourself, then run around for 10 minutes to increase circulation, and after that keep your hands warm with a heater or hot water bottle or heat pack. If you do the test after these things it should help with blood flow.